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Hydrophobic interaction chromatography. Hydrophobic interactions between proteins and the chromatographic matrix can be exploited to purify proteins. In hydrophobic interaction chromatography the matrix material is lightly substituted with hydrophobic groups. These groups can range from methyl, ethyl, propyl, octyl, or phenyl groups.
Currently offered reagents are an array of Hydrophobic Interaction Chromatography (HIC) tools for the separation and purification of proteins and other biomolecules based on their hydrophobicity. Our G-Sep™ Butyl Agarose Fast Flow (FF) and G-Sep™ Octyl Agarose Fast Flow (FF) resins are 6% cross-linked agarose with butyl or octyl groups for Hydrophobic Interaction Chromatography (HIC). These resins are used to separate proteins and biomolecules based on relative hydrophobicity.
Also available are pre-packed G-Trap™ columns for separation and purification of proteins based on hydrophobicity. Our G‐Trap™ Butyl Agarose 6 Fast Flow and G‐Trap™ Octyl Agarose 6 Fast Flow are convenient and reliable purification columns. The G‐Trap™ Phenyl Agarose 6 Fast Flow (Low Sub) and G-Trap™ Phenyl Agarose 6 Fast Flow (High Sub) expand the range of binding capacities to give researchers additional flexibility based on the properties of their protein of interest. All G-Trap™ columns are available in 1mL and 5mL sizes.
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